Partial Purification of the Extracellular Hemolysin of Pseudomonas Aeruginosa.

One-step purification and characterization of alginate lyase from a clinical Pseudomonas aeruginosa with destructive activity on bacterial biofilm

Objective(s): Pseudomonas aeruginosais a Gram-negative and aerobic rod bacterium that displays mucoid and non-mucoid phenotype. Mucoid strains secrete alginate, which is the main agent of biofilms in chronic P. aeruginosa infections, show high resistance to antibiotics; consequently, the biological disruption of mucoid P. aeruginosa biofilms is an attractive area of study for researchers. Algin...

Factors That Influence Toxigenicity of Pseudomonas Aeruginosa.

Liu, Pinghui V. (University of Louisville, Louisville, Ky.). Factors that influence toxigenicity of Pseudomonas aeruginosa. J. Bacteriol. 88:1421-1427. 1964.-Hemolysin, lecithinase, and protease of Pseudomonas aeruginosa were produced with cellophane-covered plates and completely synthetic agar of simple composition containing alpha-alanine, aspartic acid, glutamic acid, glucose, and salts. The...

Characteristics of heat-stable extracellular hemolysin from Pseudomonas aeruginosa.

Heat-stable hemolysin produced by the cellophane-agar plate method consisted of two major acidic glycolipids. One was composed of 2 mol of rhamnose and 2 mol of beta-hydroxydecanoic acid, and the other was composed of 1 mol of rhamnose and 2 mol of beta-hydroxydecanoic acid. The hemolysis-producing moiety of the hemolysin was shown to be the dimer of beta-hydroxydecanoic acid contained in the g...

Isolation, purification and partial characterization of pyocyanin and rhamnolipids from Pseudomonas aeruginosa BTRY1

EXTRACELLULAR LIPA SE ACTIVITY CHARACTERIZATION OF SOME PSEUDOMONAS AERUGINOSA STRA INS ISOLATED FROM HUMAN INFECTIONS

Pseudomonas aeruginosa EF2, ATCC 9027 and ATCC 19660 were grown in a continuous culture under Tween 80 (polyoxyethylene sorbitan monooleate) limitation and optimum conditions (pH 6.5, 37°C at dilution rate of 0.05/h). Culture supernatants were carefully removed and stored at -20°C. To purify the lipases, the culture supernatant was reduced in volume to approximately 10 mL by an ultrafiltra...